In Vitro Detection and Quantification of Botulinum Neurotoxin Type E Activity in Avian Blood

In vitro detection and quantification of botulinum neurotoxin type e activity in avian blood.

Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can t...

Detection and quantification of botulinum neurotoxin type a by a novel rapid in vitro fluorimetric assay.

Botulinum neurotoxin type A (BoNT/A), the most poisonous substance known to humans, is a potential bioterrorism agent. The light-chain protein induces a flaccid paralysis through cleavage of the 25-kDa synaptosome-associated protein (SNAP-25), involved in acetylcholine release at the neuromuscular junction. BoNT/A is widely used as a therapeutic agent and to reduce wrinkles. The toxin is used a...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

: metadiscourse in introduction sections of applied linguistics and physics research articles: exploring variation in frequency and type

abstract in written mode of language, metadiscourse markers are used commonly to help writers in general and academic writers in particular to produce coherent and professional texts. the purpose of the present study was to compare introduction sections of applied linguistics and physics articles regarding their use of interactive and interactional metadiscourse markers based on the model pro...

Toxicity of Clostridium botulinum type E neurotoxin to Great Lakes fish: implications for avian botulism.

Since 1999, large-scale mortalities of fish-eating birds have been observed on the Great Lakes, and more specifically on Lake Erie. Type E botulism has been established as the primary cause of death. The mechanism of type E botulism exposure in fish-eating birds is unclear. Given that these birds are thought to eat live fish exclusively, it seems likely that their prey play a key role in the pr...